Influence of biphasic calcium phosphate granulometry on bone ingrowth, ceramic resorption, and inflammatory reactions: Preliminaryin vitro andin vivo study

This study aims at evaluating bone growth in critical-sized femoral defects of rats filled with macro micro porous biphasic calcium phosphate ceramic (MBCP) cylinders surrounded or not by a resorbable collagen membrane. Femoral defects left empty (control) exhibited partial bone ingrowths after 3 and 6 weeks and were completely healed at 12 weeks. The defects filled with the collagen membranes appeared partially healed suggesting that the membranes constraint bone ingrowth. Bone formation occurred around the collagen membrane which partially degraded over time. In the MBCP/membrane group, bone has grown inside the macro pores of the MBCP cylinders. Bone ingrowth was more rapid and abundant in the defects filled with MBCP alone. The combination MBCP/collagen membrane may be beneficial for the reconstruction of large bone defects without using repetitive surgeries and autologous bone grafting.

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Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth

Biomaterials ◽

10.1016/s0142-9612(97)00180-4 ◽

1998 ◽

Vol 19 (1-3) ◽

pp. 133-139 ◽

Cited By ~ 452

Author(s):

Olivier Gauthier ◽

Jean-Michel Bouler ◽

Eric Aguado ◽

Paul Pilet ◽

Guy Daculsi

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

Calcium Phosphate Ceramics

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Calcium Phosphate Ceramic Blasting on Titanium Surface Improve Bone Ingrowth

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1351 ◽

2007 ◽

Vol 361-363 ◽

pp. 1351-1354 ◽

Cited By ~ 2

Author(s):

Eric Goyenvalle ◽

Eric Aguado ◽

Ronan Cognet ◽

Xavier Bourges ◽

G. Daculsi

Keyword(s):

Surface Roughness ◽

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Titanium Surface ◽

Bone Ingrowth ◽

High Surface ◽

Titanium Implants ◽

Calcium Phosphate Ceramic ◽

Abrasive Particles ◽

Roughened Surface

Surface roughness modulates the osseointegration of orthopaedic and dental titanium implants. High surface roughness is currently obtained by blasting of titanium implants with silica or aluminium abrasive particles. This process includes into the surface abrasive particles and may cause the release of cytotoxic silica or aluminium ions in the peri implant tissue. To overcome this drawback, we currently develop an innovative gridblasting process using Biphasic Calcium Phosphate (BCP) particles (RBBM Resorbable and Biocompatible Blast Media) to generate biocompatible roughened titanium surface. This work present the technique of blasting using RBBM particles to provide a roughened surface which does not release cytotoxic elements and (ii) to assess the effects of such a roughened surface for bone osteointegration in critical size rabbit defect. Our results demonstrate that resorbable biphasic calcium phosphate abrasive particles can be used to create titanium surface roughness. This grid blasting process increases surface roughness of titanium implants and offers a non cytotoxic surface for rapid and efficient osteointegration.

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Five Years Clinical Follow Up Bone Regeneration with CaP Bioceramics

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1339 ◽

2007 ◽

Vol 361-363 ◽

pp. 1339-1342 ◽

Cited By ~ 3

Author(s):

Clemencia Rodríguez ◽

Alain Jean ◽

Sylvia Mitja ◽

G. Daculsi

Keyword(s):

Calcium Phosphate ◽

Bone Regeneration ◽

Biphasic Calcium Phosphate ◽

Tooth Extraction ◽

Alveolar Bone ◽

Bone Ingrowth ◽

X Ray ◽

Dental Implantation ◽

Preventive Method

To overcome autograft use for dental implantation, it is important to prevent bone loss after tooth extraction or to restore alveolar bone level after pathological diseases. Biphasic calcium phosphate (BCP), mixture of HA and ß-TCP, have proven its performance in orthopaedic, while few studies have been reported in dentistry. We reported 5 years clinical follow up on bone regeneration after immediate dental root filling. MBCP 60/40 and MBCP 20/80 are biphasic CaP intimate mixture of HA/TCP 60/40 and 20/80; with interconnected macroporosity and microporosity. Forty cases have been distributed in two groups for alveolar pocket filling. Seven cases without filling are used as control. X-Ray at 0, 3, 6, 12 months and 5 years follow up for some patients were performed. In all the 40 cases, radio-opacity of the implantation area decreases on time, indicating resorption and bone ingrowths at the expense of the two bioceramics. No difference in the resorption kinetics appeared on X-Ray. After 1 year, the implantation area looks as physiological bone and is maintained on time. The newly formed bone is preserved after 5 years contrarily to the controls cases (without filling)where we observed decrease of 2 to 5 mm. This study demonstrated that immediate filling of alveolar pocket after tooth extraction is a preventive method of the jaw bone resorption. After long term (other one year) resorption and bone ingrowth were demonstrated for both micro and macroporous biphasic calcium phosphate with two different HA/TCP ratio.

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In Vivo Performance of an Injectable Biphasic Calcium Phosphate Bone Filler

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.396-398.583 ◽

2008 ◽

Vol 396-398 ◽

pp. 583-586

Author(s):

Pierre Layrolle ◽

Serge Baroth ◽

Eric Goyenvalle ◽

Eric Aguado ◽

Françoise Moreau ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Healing ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

Submicron Particles ◽

Bone Filler ◽

Osteoclastic Resorption ◽

Tcp Phases

An hydrated putty was prepared by mixing submicron particles, rounded particles and granules of Biphasic Calcium Phosphate (BCP) ceramics composed of HA and β-TCP phases. The material filled entirely critical sized defects in the femoral epiphysis of NZW rabbits. After 3, 6 and 12 weeks, histology revealed that submicron particles were rapidly degraded by multinucleated TRAP-positive cells. This osteoclastic resorption stimulated bone ingrowth while the large BCP particles served as scaffold supporting bone healing by osteoconduction.

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Porous Biphasic Calcium Phosphate for Biomedical Application

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.49.101 ◽

2021 ◽

Vol 49 ◽

pp. 101-110

Author(s):

Wafaa A. Hussain ◽

Entessar H.A. Al-Mosawe ◽

Mukhlis M. Ismail ◽

Luay H. Alwan

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Pathogenic Bacteria ◽

Biomedical Application ◽

Bone Ingrowth ◽

Physical And Mechanical Properties ◽

Apparent Porosity ◽

X Ray ◽

Natural Bone ◽

Fast Bone

Excellent osteoconductivity and resorbability achieved when porous bioceramics have highsurface area that providing fast bone ingrowth. Porous samples were fabricated by using biphasic calcium phosphate BCP (achieved from HA heat treated at 850 oC) with 10 and 20 wt% of ovalbumin binder powder and mixture of carrot fibers and ovalbumin powders (1:1) then dried at 60oC and fired at 1300 oC. Structural, physical and mechanical properties of the prepared porous bioceramic were determined involved X-ray diffraction, Fourier transform infrared spectroscopy FTIR, apparent porosity, water absorption, apparent solid density and compressive strength. The results of X-ray and FTIR showed that the heat treatment of HA was succeeded in forming biphasic calcium phosphate. The apparent porosity values increased with increasing of the binder and carrot fibers content and the growths density of bacteria on bioceramics are less than natural bone. The effect of pathogenic bacteria (Pseudomonas & Staphylococcus) that cause pollution on porous calcium phosphate and natural bone (Albino mice) has been studied.

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Macroporous Biphasic Calcium Phosphate Bioceramics Wedges in High Tibial Osteotomy

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.1319 ◽

2007 ◽

Vol 361-363 ◽

pp. 1319-1322

Author(s):

Jean Louis Rouvillain ◽

F. Lavallé ◽

Hugues Pascal-Mousselard ◽

Yves Catonné ◽

Olivier Delattre ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

Tibial Osteotomy ◽

X Rays ◽

Histological Data ◽

Medial Gonarthrosis ◽

One Year ◽

Large Bone

In this study, we report human clinical and histological data on the use of micromacroporous biphasic calcium phosphate wedges in combination with osteosynthesis with adjustable screws for open tibial osteotomy. 42 patients were operated for open tibial osteotomy for valgisation due to incipient medial gonarthrosis. The population was composed of 43 knees (25 right knees and 18 left knees) in 13 women and 29 men, with a mean age of 46 years. Radiological follow-up at D+1, D+90 and D+360, and 12 biopsies for histology were processed after the removal of the osteosynthesis. Consolidation was obtained in 100 % of cases (except one, because of failure of the osteosynthesis). Regular bone ingrowth was observed on X-ray, with maintain of the corrections at one year in 98.5 % of cases. Only 8 % of cases showed a radiolucent interface after D+360. Histological examinations demonstrated resorption of the MBCP and large bone ingrowth. Polarised microscopy confirmed the lamellar bone, and the X-rays and microscanner revealed well mineralised and organised newly-formed bone.

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Performance for Bone Ingrowth of Biphasic Calcium Phosphate Ceramic Versus Bovine Bone Substitute

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.1379 ◽

2006 ◽

Vol 309-311 ◽

pp. 1379-1382 ◽

Cited By ~ 2

Author(s):

G. Daculsi ◽

P. Corre ◽

Oliver Malard ◽

Racquel Z. LeGeros ◽

Eric Goyenvalle

Keyword(s):

Calcium Phosphate ◽

Bone Substitute ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

Maxillofacial Surgery ◽

Bovine Bone ◽

Calcium Phosphate Ceramic ◽

Porous Biphasic Calcium Phosphate

Calcium phosphate bioceramics and bovine bone xenograft with or without sintering are more or less used in orthopaedics or in maxillofacial surgery. In this study we compare in a rat femoral epiphysis model after 3 weeks of implantation the bone in growth at the expense of granules of same size of micro macro porous biphasic calcium phosphate MBCP, sintered bovine bone and unsintered BioOss.

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Effects of Na Alginate in the Porosity of Scaffold Biphasic Calcium Phosphate/Alginate Composites

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.696.183 ◽

2016 ◽

Vol 696 ◽

pp. 183-186

Author(s):

Kiagus Dahlan ◽

Nur Aisyah Nuzulia ◽

Setyanto Tri Wahyudi ◽

Setia Utami

Keyword(s):

Calcium Phosphate ◽

Pore Size ◽

Bone Grafting ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

Ct Scanning ◽

Porous Scaffolds ◽

X Ray Diffraction ◽

Compact Structure ◽

Bone Grafting Materials

Biphasic calcium phosphate (BCP) bioceramics containing hydroxyapatite and β-Tricalcium Phosphate phases is one of bone grafting biomaterials that quite often used in bone regeneration due to its best characteristics in biodegradability and stability. Nevertheless, those properties are less enhance the bone ingrowth because of the dense and compact structure. Therefore, one of promising way to improve those properties is fabricating the bioceramics in the form of porous scaffolds. In this research BCP/alginate composite scaffolds were synthesized by using calcined chicken’s eggshells as the calcium donor. The synthesis of BCP/alginate scaffolds was performed by adding Na-alginate into BCP bioceramics as a matrix and porosifier with various percentage wt% of 20%, 30% and 40% using freeze drying processes. X-ray diffraction (XRD) results showed that the addition of alginate tends to decrease the crystallinity of BCP/alginate composites. This XRD result, shown by peaks in 2θ range of 10o-20o, is in accordance with fourier transform infrared spectra showing the characteristics of functional groups of BCP and alginate. Moreover, the obtained mean pore size of samples are in the range of trabecular bone pore size which is about 200-400 μm with highest porosity more than 65% presented by μ-CT scanning analysis. These results indicate that the corresponding composites may suit as ideal bone grafting materials.

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Prospective Study of the Mastoid Plasties Using Resorbable Bioceramic MBCP® in Surgery of Cholesteatoma

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.720.69 ◽

2016 ◽

Vol 720 ◽

pp. 69-73

Author(s):

Hugo Dercourt ◽

Charles Henri A. Le Thuaut ◽

Guillaume Michel ◽

K Buquet ◽

G. Daculsi ◽

...

Keyword(s):

Pilot Study ◽

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Bone Ingrowth ◽

High Rate ◽

Mastoid Cavity ◽

Histopathological Analysis ◽

Sample Collection ◽

Closed Technique ◽

Nose And Throat

Tympanoplasty is the reference for cholesteatoma treatment in ear, nose, and throat (ENT surgery), with the realization of a closing technique, which preserves the external auditory canal. An insufficient pneumatisation is an element favoring the occurrence and recurrence of cholesteatoma. In a prospective clinical pilot study, we have realized the reconstruction of the mastoid cortical bone by a Micro Macroporous Biphasic Calcium Phosphate bioceramics disks (MBCPTM Biomatlante France) using the closed technique, in order to maintain or increase the mastoid pneumatisation. Eleven patients were used for this study, and some of them have a sample collection for histomorphometry and histopathological analysis. Bone regeneration at the expense of the bioceramic was observed. The size and shape of the disk was not preserved due to the large resorption and bone ingrowth at the expense of the implant. However 6 patients presented cholesteatoma recurrence. The MBCPTM disks are able to reconstruct the mastoid cortical and maintain the mastoid pneumatisation contrarily to the technique of filling the mastoid cavity. However, the high rate of cholesteatoma recurrence observed in our study, confirmed the interest to fill mastoid that suppress airspace posterior cavities.

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